Journal: Journal of Cellular and Molecular Medicine

Article Title: Tuina Inhibits Synaptic Plasticity Through the Astrocytic NDRG2 / GLT ‐1 Pathway to Alleviate Neuropathic Pain

doi: 10.1111/jcmm.71013

Figure Lengend Snippet: AAV‐NDRG2 weakens Tuina's effect in promoting glutamate uptake and synaptic plasticity in CCI rats. (A) Protein expression levels of ATP1A1 and ATP1B1 in SDH. GAPDH was used as the internal control. (B, C) Relative protein expression levels of ATP1A1 (B) and ATP1B1 (C) in each group. n = 4. (D) Pearson's correlation of glutamate and γ ‐aminobutyric acid levels with ATP1A1 and ATP1B1 protein expression. (E, F) Concentrations of glutamate and γ ‐aminobutyric acid in each group. n = 4. (G) Quantification analysis of the number of VGLUT1/PSD95 in SDH. n = 4. (H) Representative images of IF analysis of VGLUT1 (blue), PSD95 (pink) and Merge (white) in SDH among groups. Scale bar, 20 μm (top), 10 μm (bottom). Data are presented as mean ± SD * p < 0.05, ** p < 0.01 versus CCI + Vector group. # p < 0.05, ## p < 0.01 versus CCI + Vector+Tuina group.

Article Snippet: Primary antibodies against NDRG2 (Abcam; ab174850, 1:100), GLT‐1 (Proteintech; 22,515–1‐AP, 1:100), GFAP (Abcam; ab7260, 1:5000), VGLUT1 (CST, 47181S, 1:200) and PSD95 (CST, 36233S, 1:100) were added to deparaffinised and blocked L4‐L6 SDH tissue sections.

Techniques: Expressing, Control, Plasmid Preparation